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KMID : 0380619900220060711
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Korean Journal of Food Science and Technology
1990 Volume.22 No. 6 p.711 ~ p.715
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Continuous Separation of Lysozyme from Egg White by Ion Exchange Column Chromatography
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최춘언/Choi, Chun Un
안태회/김현위/박기문/박성준/김현석/Ahn, Tae Hoe/Kim, Hyean Wee/Park, Ki Moon/Park, Seong Joon/Kim, Hyeon Seok
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Abstract
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Continuous column chromatographic separation of lysozyme from egg white was investigated. A weak acid type ration exchange resin, Duolite C-464, was used because of high lysozyme recovery and ease of column operation in this experiment. The resin was equilibrated at pH 7.9±0.1 in Na + form. Continuous lysozyme separation was processed by repeating cycles(one cycle : resin equilibration, flow egg white, rinse, lysozyme elution)in automated preparative Liquid Chromatography(LC) system(column size ; i.d. 50 mm, resin bed volumn : 1020 ml). At comparison of UV levels in rinse end point and elution end point of every cycle, the LJV levels of rinse end point are maintained below 30% for 19 cycles and that of elution end point are also maintained below 30% for 17 cycles, stably, but was increased above 50% after 18 cycle. That indicated the eluting ability of lysozyme was reduced conspicuously after 18 cycle in continuous cycling process. The recovery of lysozyme was maintained above 90% from one to 17 cycle, but was decreased to 72% and 65% in 18 cycle and 19 cycle, respectively.
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KEYWORD
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